Graduation Level Topics

 

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1. Production of Amylase Enzymes from Fungal Sources

Graduation students can cultivate Aspergillus niger on starch-based media to produce extracellular amylases. Enzyme activity is assayed using iodine-starch tests and DNS method for reducing sugars. This project reflects India’s food and textile industries, where amylases are widely used for starch hydrolysis.

2. Optimization of Protease Production Using Submerged Fermentation

Students can grow Bacillus subtilis in nutrient-rich broth to produce proteases. Different parameters like pH, temperature, and substrate concentration are tested to optimize yields. Proteases are crucial in India’s detergent and leather industries, making this project highly practical.

3. Enzymatic Hydrolysis of Milk Lactose Using Immobilized Lactase

Graduation projects can involve immobilizing lactase on alginate beads and using it to hydrolyze lactose in milk. Glucose and galactose production is monitored spectrophotometrically. This project introduces enzyme immobilization, directly relevant in India’s dairy sector producing lactose-free milk.

4. Extraction of Cellulases from Agricultural Residues

Students can grow cellulolytic fungi on rice straw or sugarcane bagasse. Enzyme activity is tested using CMC assays. This project links waste utilization with enzyme production, relevant to India’s agricultural economy.

5. Application of Lipases in Biodiesel Production

Graduation students can use microbial lipases to catalyze transesterification of vegetable oils into biodiesel. Fuel properties like viscosity and calorific value are measured. This project integrates enzyme technology with renewable energy, which India is expanding under its biofuel policy.

6. Screening Soil Microorganisms for Enzyme Production

Students can isolate microbes from soil samples and screen them for enzyme activity using plate assays (e.g., starch agar for amylase, skim milk agar for protease). This project introduces basic microbial biotechnology, already applied in Indian academic labs.

7. Use of Pectinase in Fruit Juice Clarification

Graduation projects can involve extracting pectinases from fungi and applying them to fruit juice. Clarity and viscosity are measured before and after treatment. This project reflects India’s fruit processing industry where pectinases are routinely applied.

8. Immobilization of Enzymes on Low-cost Supports

Students can test immobilization of enzymes such as amylase or lipase on supports like clay beads, coconut shell powder, or biochar. Enzyme activity and reusability are compared with free enzymes. This introduces cost-effective enzyme technology, relevant in India’s small-scale industries.

9. Alkaline Proteases for Leather Industry Applications

Graduation students can extract alkaline proteases from Bacillus species and test them for dehairing animal hides. Efficiency and quality are compared with chemical treatments. This project mirrors enzyme-based eco-friendly leather processing, already being promoted in Indian tanneries.

10. Enzymatic Detergent Formulations Using Microbial Enzymes

Students can formulate detergents supplemented with proteases and amylases, then test stain removal efficiency on fabric samples. Enzyme stability in detergent formulations is also analyzed. This project reflects India’s detergent industry, which is a major consumer of industrial enzymes.

11. Enzyme-assisted Desizing of Cotton Fabrics

Graduation students can apply amylases to remove starch-based sizing agents from cotton fabrics. Fabric strength and texture are tested post-treatment. This project introduces enzyme applications in textiles, a sector where Indian mills are increasingly using eco-friendly enzymatic desizing.

12. Extraction of Chitinase from Fungal Cultures

Students can cultivate fungi on chitin-rich substrates like crab shell powder and extract chitinases. Enzyme activity is measured through N-acetylglucosamine release. This project links enzyme biotechnology to biocontrol, useful in India’s agricultural biopesticide programs.

13. Production of Xylanase for Paper Pulp Bleaching

Graduation projects can involve cultivating Trichoderma reesei or Aspergillus species to produce xylanase. The enzyme is applied to kraft pulp to reduce lignin content. This project demonstrates enzymatic bleaching, already adopted by Indian paper industries to reduce chlorine use.

14. Use of Enzymes for Bio-polishing of Textiles

Students can apply cellulases to cotton fabrics for bio-polishing, improving softness and reducing pilling. Fabric quality is assessed using microscopy. This project reflects real applications in India’s textile export industry where bio-polishing adds commercial value.

15. Enzyme-assisted Extraction of Plant Oils

Graduation projects can test enzyme cocktails (cellulases, hemicellulases, pectinases) to enhance oil yield from oilseeds. Yield is compared with solvent extraction. This project demonstrates greener extraction technologies, relevant to India’s edible oil industry.

16. Production of Glucoamylase for Glucose Syrup Preparation

Students can grow Aspergillus niger under submerged fermentation to produce glucoamylase. Starch hydrolysis to glucose is measured by DNS assay. This project reflects Indian sweetener and confectionary industries where glucose syrup is a major product.

17. Use of Phytase in Poultry Feed Improvement

Graduation projects can involve producing phytase from Bacillus species and testing its effect on phosphorus availability in poultry feed. Nutrient digestibility is measured in feeding trials. This project is relevant to India’s poultry industry, where phytase reduces feed costs and improves sustainability.

18. Screening Marine Microbes for Salt-tolerant Enzyme Production

Students can isolate halophilic bacteria from marine water samples and test them for production of salt-tolerant proteases or lipases. This project highlights India’s coastal biodiversity as a source for industrial enzymes.

19. Enzymatic Clarification of Sugarcane Juice

Graduation students can apply dextranase to raw sugarcane juice to break down polysaccharides and improve clarity. Treated juice is tested for purity and crystallization properties. This project is highly relevant in India’s sugar industry, which relies heavily on enzymatic processing.

20. Immobilization of Invertase for Continuous Production of Invert Sugar

Students can immobilize invertase on calcium alginate beads and use it in column reactors to hydrolyze sucrose solutions. Continuous production efficiency and enzyme stability are measured. This project reflects India’s confectionary and beverage industry where invert sugar is widely used.

21. Application of Lactase in Dairy Product Development

Graduation students can test lactase enzyme for hydrolyzing lactose in milk to produce lactose-free milk. Glucose and galactose concentrations are monitored enzymatically. This project is directly relevant in India’s dairy sector where lactose-free products are gaining popularity.

22. Use of Catalase in Textile Bleaching Wastewater Treatment

Students can apply catalase to degrade residual hydrogen peroxide in textile bleaching effluents. Treated water is tested for peroxide removal and reusability. This project links enzymes to eco-friendly wastewater management, already adopted in Indian textile clusters.

23. Enzymatic Dehairing of Goat Skins Using Proteases

Graduation projects can test alkaline proteases for dehairing goat skins, comparing with traditional lime-sulfide treatments. Quality of leather and environmental impact are assessed. This project reflects India’s leather industry shift toward enzyme-based cleaner technologies.

24. Screening Thermophilic Bacteria for Heat-stable Enzyme Production

Students can isolate bacteria from hot springs or compost and test for thermostable amylase or protease production. Enzyme activity at high temperatures is measured. This project is significant in India, where thermostable enzymes are valuable for industrial food processing.

25. Use of Papain in Meat Tenderization

Graduation students can extract papain from papaya latex and test it for tenderizing tough meat cuts. Tenderness is measured using shear force analysis. This project is relevant in India’s meat industry, where papain is widely applied for texture improvement.

26. Application of Glucose Oxidase in Food Preservation

Students can use glucose oxidase to reduce oxygen in food packaging systems, extending shelf-life. Oxygen levels and microbial growth are monitored in stored foods. This project introduces enzymatic food preservation, used in India’s bakery and dairy industries.

27. Enzymatic Saccharification of Lignocellulosic Biomass

Graduation projects can involve enzymatic hydrolysis of rice husk or bagasse using cellulases and hemicellulases. Reducing sugar yield is quantified. This project connects enzyme biotechnology with India’s bioethanol industry using agricultural residues.

28. Use of Keratinase for Poultry Feather Waste Management

Students can cultivate keratinase-producing microbes on poultry feathers and measure degradation efficiency. Hydrolysates are tested as animal feed supplements. This project reflects India’s poultry sector, where feather waste is abundant and needs value addition.

29. Application of Peroxidase in Dye Degradation from Textile Effluents

Graduation students can use horseradish peroxidase or microbial peroxidases to degrade azo dyes. Decolorization efficiency is measured spectrophotometrically. This project is highly relevant to Indian textile hubs where enzymatic dye removal reduces chemical pollution.

30. Enzymatic Production of Cheese Using Microbial Rennet

Students can use microbial rennet enzymes for milk coagulation and cheese production. Texture and yield are compared with calf rennet. This project supports India’s dairy industry, which is transitioning to microbial rennet due to ethical and supply concerns.

31. Application of β-galactosidase in Prebiotic Production

Graduation students can use β-galactosidase to produce galacto-oligosaccharides (GOS) from lactose. Product yield is measured by HPLC. This project reflects India’s dairy and nutraceutical industries where GOS is gaining importance as a functional food ingredient.

32. Enzyme-assisted Bioethanol Production from Molasses

Students can apply glucoamylase and invertase to hydrolyze molasses sugars for ethanol fermentation. Fermentation efficiency is monitored with yeast. This project links enzymes to India’s sugarcane ethanol program, central to the national biofuel policy.

33. Application of Pectinase in Tea Fermentation

Graduation projects can test microbial pectinases for improving tea leaf fermentation, enhancing flavor and clarity. Sensory quality is evaluated after treatment. This project is relevant to India’s tea industry, a major global exporter.

34. Extraction of Laccase for Bioremediation of Pesticides

Students can cultivate white-rot fungi to produce laccases and apply them to pesticide-contaminated water. Degradation efficiency is measured using spectrophotometry. This project is practical in India where pesticide pollution affects agricultural ecosystems.

35. Use of Protease in Brewery By-product Valorization

Graduation students can apply proteases to hydrolyze protein-rich brewery waste into amino acid-rich hydrolysates. Nutrient value is analyzed for animal feed. This project demonstrates waste valorization, relevant for India’s growing brewery sector.

36. Enzymatic Deinking of Waste Paper Using Cellulase-Xylanase Cocktails

Students can apply enzyme cocktails to recycled paper pulp and test for ink removal efficiency. Brightness and strength of treated pulp are measured. This project is relevant to Indian paper industries seeking eco-friendly recycling methods.

37. Application of α-Amylase in Baking Industry

Graduation projects can involve testing amylase supplementation in bread-making for improved softness and shelf-life. Texture and staling rate are monitored. This project connects enzyme use to India’s bakery sector, where demand for quality bread is increasing.

38. Use of Alkaline Lipases for Detergent Stability Studies

Students can extract alkaline lipases from Bacillus species and test their stability in detergent formulations under various conditions. Stain removal efficiency is compared with chemical detergents. This project supports India’s detergent enzyme market.

39. Application of Enzymes for Wine Clarification

Graduation students can apply pectinases and cellulases to grape juice during fermentation, improving clarity and yield. Final product is analyzed for turbidity and taste. This project links enzyme biotechnology to India’s emerging wine industry.

40. Enzymatic Treatment of Coir Fiber for Quality Improvement

Students can apply cellulases and pectinases to coir fibers to enhance softness and dye uptake. Fiber quality is tested before and after treatment. This project is relevant in India’s coir industry, particularly in Kerala and Tamil Nadu.

41. Enzyme-assisted Brewing of Low-alcohol Beer

Graduation students can apply amyloglucosidase to adjust fermentable sugars, producing low-alcohol beer. Alcohol content and flavor are analyzed post-fermentation. This project links enzymes to India’s expanding craft brewing sector where new product lines are emerging.

42. Application of Transglutaminase in Meat Product Development

Students can use microbial transglutaminase to improve binding and texture in processed meat products. Gel strength and sensory properties are evaluated. This project reflects India’s growing meat processing industry, which applies enzyme technologies for product innovation.

43. Enzyme-assisted Extraction of Natural Dyes from Plant Sources

Graduation projects can test cellulases and pectinases for enhancing dye yield from sources like turmeric or hibiscus. Dye strength and fabric application are studied. This project connects enzyme technology with India’s natural dye and textile industries.

44. Production of Cold-active Enzymes from Psychrotrophic Microbes

Students can isolate psychrotrophic microbes from Himalayan soils and screen them for cold-active amylases or proteases. Enzyme activity at low temperatures is analyzed. This project reflects India’s biodiversity-based enzyme exploration.

45. Application of Urease in Agricultural Soil Testing

Graduation projects can involve using microbial urease to hydrolyze urea in soil samples for rapid nitrogen estimation. Ammonia release is quantified. This project is practical for India’s agricultural extension services to improve fertilizer management.

46. Enzyme-assisted Production of Herbal Extracts

Students can apply cellulases and pectinases to enhance extraction of bioactive compounds from medicinal plants. Yield and antioxidant activity are measured. This project supports India’s Ayurvedic and herbal industry, where enzymatic extraction is replacing chemical solvents.

47. Application of Enzyme Cocktails in Animal Feed Digestibility

Graduation students can supplement poultry or cattle feed with cellulases, amylases, and proteases to improve nutrient digestibility. Feed trials are conducted, and growth performance is analyzed. This project is widely relevant to India’s livestock sector.

48. Enzymatic Hydrolysis of Whey for Value-added Products

Students can apply proteases to hydrolyze whey proteins into bioactive peptides. Antioxidant or antimicrobial properties are tested. This project adds value to whey, a by-product abundantly generated in India’s dairy industry.

49. Enzyme-assisted Bioplastic Production from Starch

Graduation projects can involve enzymatic hydrolysis of starch into lactic acid, followed by polymerization into polylactic acid (PLA). Biodegradability tests are performed. This project connects enzyme biotechnology with India’s initiatives on sustainable plastics.

50. Application of Enzyme-based Biosensors for Glucose Detection

Students can design biosensors using immobilized glucose oxidase on electrodes. Performance is tested with standard glucose solutions and real samples. This project introduces enzymatic biosensors, relevant for India’s healthcare and food quality monitoring.

Post-Graduation Level Topics

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1. Protein Engineering of Amylases for High-temperature Stability

Postgraduates can apply site-directed mutagenesis to alter amino acids in α-amylase for enhanced thermostability. Mutant enzymes are expressed, purified, and tested for activity under industrial conditions. This project reflects India’s food and textile industries, which require robust enzymes for large-scale starch processing.

2. Development of Recombinant Proteases Using Expression Systems

Students can clone protease genes from Bacillus into E. coli or Pichia pastoris for large-scale recombinant production. Yield and enzyme properties are compared with native strains. This project supports India’s detergent and leather industries where demand for recombinant enzymes is rising.

3. Directed Evolution for Improved Lipase Catalytic Efficiency

Postgraduates can perform random mutagenesis and screening of lipase variants for enhanced activity in organic solvents. Selected mutants are sequenced to identify beneficial mutations. This project connects synthetic biology with biodiesel production, already under study in Indian bioenergy labs.

4. Immobilization of Enzymes in Nanomaterials for Industrial Use

Students can immobilize cellulases or lipases on nanostructured supports such as silica nanoparticles or carbon nanotubes. Activity, stability, and reusability are tested. This advanced project aligns with Indian research efforts to reduce enzyme costs in continuous processes.

5. Metagenomic Mining of Novel Enzymes from Extreme Environments

Postgraduates can extract DNA from hot springs, saline lakes, or compost and construct metagenomic libraries to identify novel hydrolases. Enzymes are expressed heterologously and tested for industrial traits. This project taps into India’s rich biodiversity for enzyme discovery.

6. Development of Enzyme-based Biosensors for Industrial Fermentation Monitoring

Students can design biosensors using immobilized oxidases or dehydrogenases for real-time monitoring of glucose or ethanol in fermentation tanks. Data integration with process control is studied. This project reflects India’s biotech fermentation industries, where process automation is expanding.

7. Enzymatic Conversion of Lignocellulosic Biomass into Platform Chemicals

Postgraduates can use cellulases, hemicellulases, and accessory enzymes to release sugars from rice husk, then ferment them into lactic acid or ethanol. This project is directly relevant to India’s bioeconomy strategies focusing on agri-waste valorization.

8. Computational Modeling and Molecular Docking for Enzyme-substrate Interactions

Students can perform molecular docking of industrial enzymes with target substrates using bioinformatics software. Binding energies and key residues are identified for rational protein design. This project brings computational biotechnology into enzyme R&D, increasingly practiced in India.

9. Development of Thermophilic Enzymes for Textile Industry Applications

Postgraduates can isolate thermophilic bacteria from hot environments, sequence their genomes, and express thermostable cellulases for bio-polishing textiles. Enzyme efficiency at high temperatures is tested on cotton fabrics. This project reflects India’s textile hubs seeking energy-efficient enzyme processes.

10. Continuous Reactor Design Using Immobilized Enzymes

Students can design packed-bed or membrane bioreactors using immobilized amylase or invertase for continuous sugar hydrolysis. Operational stability, productivity, and cost-effectiveness are evaluated. This project reflects industrial scaling needs in India’s sugar and beverage industries.

11. CRISPR-Cas9 Mediated Strain Improvement for Enzyme Overproduction

Postgraduates can apply CRISPR-Cas9 to knock out repressor genes or integrate multiple enzyme copies in microbial strains. Mutants are evaluated for enzyme yield and stability. This project is highly relevant in India’s enzyme manufacturing sector, where genome editing is being explored for industrial-scale production.

12. Co-expression of Multiple Enzymes for Biomass Saccharification

Students can engineer yeast or fungi to co-express cellulases, xylanases, and β-glucosidases in a single host. Hydrolytic efficiency on lignocellulosic substrates is measured. This approach is under research in India to reduce enzyme cocktail costs in biofuel industries.

13. Enzyme Engineering for Organic Solvent Tolerance

Postgraduates can evolve lipases or proteases to maintain activity in organic solvents, useful for biodiesel and pharmaceutical applications. Mutants are screened under solvent stress conditions. This project supports India’s growing pharmaceutical enzyme sector.

14. High-cell Density Fermentation for Recombinant Enzyme Production

Students can optimize fed-batch fermentation strategies for E. coli or Pichia pastoris to maximize enzyme yield. Biomass productivity and enzyme recovery are monitored. This project mirrors India’s biotech industries scaling up fermentation for enzyme manufacturing.

15. Application of Enzymes in Bioplastic Degradation

Postgraduates can test cutinases or PETases for enzymatic degradation of polyethylene terephthalate (PET) plastics. Breakdown products are analyzed using HPLC. This project aligns with India’s plastic waste management policies encouraging bio-based solutions.

16. Structural Bioinformatics for Rational Enzyme Redesign

Students can model enzyme 3D structures and perform in silico mutagenesis to predict stability improvements. Designed variants are expressed and validated experimentally. This project reflects India’s rising expertise in computational biology applied to enzyme research.

17. Application of Enzymes in Green Chemistry Catalysis

Postgraduates can explore enzymatic alternatives for chemical reactions such as esterification, oxidation, or peptide synthesis. Reaction efficiency and eco-friendliness are compared with chemical catalysts. This project supports India’s pharmaceutical and fine chemical industries moving toward sustainable catalysis.

18. Multi-enzyme Immobilization for Cascade Reactions

Students can immobilize sequential enzymes on nanostructured supports for performing multi-step reactions in a single reactor. Substrate-to-product conversion efficiency is studied. This project mirrors integrated biocatalyst systems being developed in India for value-added chemical production.

19. Application of Extremozymes in Detergent Formulations

Postgraduates can test enzymes isolated from hot springs or saline lakes for their performance in detergents at extreme pH and temperatures. Stability and stain removal are compared to commercial enzymes. India’s detergent industries are already exploring extremozymes for new formulations.

20. Bioreactor Scale-up Strategies for Industrial Enzyme Production

Students can compare lab-scale enzyme fermentation data with pilot-scale stirred tank and airlift reactors. Parameters such as oxygen transfer, mixing, and productivity are optimized. This project reflects Indian enzyme companies scaling up bioreactor processes for commercial enzyme supply.

21. Whole-genome Sequencing of Industrial Enzyme-producing Microbes

Postgraduates can sequence the genomes of Aspergillus or Bacillus strains used for enzyme production. Annotation helps identify genes linked to enzyme secretion and stress tolerance. This project supports Indian enzyme companies that are adopting genomics for strain improvement.

22. Development of Thermostable Phytases for Poultry Feed

Students can engineer phytases to resist pelleting temperatures in poultry feed production. Enzyme activity is tested post-pelletization. This project is crucial in India’s poultry industry, where phytases reduce feed costs and improve nutrient utilization.

23. Comparative Proteomics of Enzyme-secreting Microorganisms

Postgraduates can use 2D gel electrophoresis and mass spectrometry to analyze secretomes of fungi producing cellulases. Differences under varying fermentation conditions are mapped. This project introduces proteomics in enzyme biotechnology, actively explored in Indian CSIR and ICAR labs.

24. Application of Cold-active Enzymes in Frozen Food Processing

Students can isolate cold-active lipases or amylases from psychrophilic microbes. Enzyme activity is tested at refrigeration temperatures. This project is relevant to India’s frozen food and seafood industries, where mild-temperature processing improves quality.

25. Nanobiocatalysts for Enhanced Enzyme Stability

Postgraduates can conjugate enzymes with metal nanoparticles or polymer nanostructures to enhance thermal and pH stability. Catalytic efficiency and reusability are compared with free enzymes. This project links nanotechnology with enzyme applications, a growing area of research in India.

26. Synthetic Biology Approaches for Novel Enzyme Pathways

Students can design synthetic operons combining enzyme genes from multiple organisms to create novel biosynthetic pathways. Expression in microbial hosts is validated. This project reflects global trends in synthetic biology, gradually expanding into Indian enzyme research labs.

27. Enzyme-assisted Bioethanol Production from Agricultural Waste

Postgraduates can optimize enzyme cocktails for hydrolyzing rice straw or sugarcane bagasse, followed by fermentation into ethanol. Process efficiency and cost analysis are included. This project supports India’s 2G bioethanol plants that depend on enzymatic saccharification.

28. Immobilized Enzymes in Continuous-flow Microreactors

Students can test immobilized lipases or proteases in microreactor setups for continuous product synthesis. Reaction efficiency and operational stability are studied. This project reflects advanced bioreactor technologies, now entering Indian enzyme research.

29. Application of Enzymes in Wastewater Bioremediation

Postgraduates can apply laccases, peroxidases, or esterases to degrade dyes, pesticides, or pharmaceutical residues. Pollutant reduction is measured via chromatography. This project addresses India’s urgent need for enzyme-based eco-remediation in industrial effluents.

30. Comparative Transcriptomics of Enzyme-producing Fungi

Students can perform RNA-seq on fungi grown under different carbon sources to identify gene expression linked to enzyme secretion. Differential expression analysis provides molecular insights. This project introduces advanced transcriptomics, actively applied in Indian biotechnology research.

31. Rational Design of Proteases for Eco-friendly Leather Processing

Postgraduates can modify protease active sites to enhance specificity for collagen degradation while minimizing damage to hides. Enzyme-treated leather is compared with chemically processed samples. This project supports India’s leather hubs, which are shifting toward greener enzyme-based processes.

32. High-throughput Screening of Mutant Enzyme Libraries

Students can use robotic platforms and colorimetric assays to screen thousands of mutant lipase or cellulase variants for improved traits. Selected mutants are validated through sequencing. This project mirrors global enzyme R&D trends, now entering Indian biotech labs.

33. Industrial Enzyme Production from Agro-industrial Wastes

Postgraduates can cultivate fungi or bacteria on substrates like wheat bran, molasses, or oil cakes for enzyme production. Fermentation yield and cost savings are compared with synthetic media. This project is highly relevant in India, where waste valorization reduces enzyme production costs.

34. Comparative Analysis of Solid-state vs. Submerged Fermentation

Students can grow Aspergillus on agro-wastes (SSF) and liquid media (SmF) to compare enzyme yield and stability. Productivity and cost-efficiency are evaluated. This project reflects Indian industries’ preference for SSF in low-cost enzyme production.

35. Enzymatic Production of High-fructose Corn Syrup (HFCS)

Postgraduates can use sequential amylase and glucose isomerase treatment to convert starch into HFCS. Yield is measured via HPLC analysis. This project supports India’s beverage and confectionery industries, where HFCS demand is growing.

36. Genome Editing for Hyper-secreting Fungal Strains

Students can use CRISPR tools to enhance secretion pathways in fungi such as Trichoderma reesei. Mutants are validated for improved cellulase output. This project is highly relevant to India’s bioethanol sector, which requires high-yield enzymes.

37. Development of Enzyme-based Bioplastics

Postgraduates can design enzymatic polymerization systems using lipases to synthesize biodegradable polyesters. Material properties are tested for strength and degradation. This project supports India’s transition toward sustainable packaging under plastic-ban policies.

38. Computational Fluid Dynamics (CFD) for Bioreactor Optimization

Students can simulate mixing, oxygen transfer, and substrate distribution in enzyme fermentation bioreactors using CFD software. Predictions are validated against experimental data. This project integrates engineering and biotechnology, increasingly applied in Indian scale-up processes.

39. Multi-omics Integration for Enzyme Yield Optimization

Postgraduates can combine genomics, transcriptomics, proteomics, and metabolomics data to map bottlenecks in microbial enzyme production. Systems biology tools are applied to optimize metabolic pathways. This project mirrors cutting-edge industrial research now entering Indian biotech labs.

40. Application of Enzymes in Textile Dye Degradation at Pilot Scale

Students can scale up peroxidase and laccase-based dye degradation in textile effluent treatment plants. Pilot-scale tests are conducted in Indian textile hubs like Tirupur. This project directly links enzyme biotechnology to industrial wastewater treatment.

41. Development of Extremozymes from Indian Hot Springs and Saline Lakes

Postgraduates can collect environmental samples from Himalayan hot springs or Rann of Kutch saline regions and screen microbes for novel extremozymes. Enzyme stability is tested under harsh industrial conditions. This project taps into India’s biodiversity for unique industrial enzymes.

42. Enzyme Engineering for Enhanced Enantioselectivity in Drug Synthesis

Students can modify lipases or oxidoreductases for stereospecific synthesis of chiral drug intermediates. Reaction yield and purity are evaluated. This project reflects India’s pharmaceutical industry’s need for biocatalysts in cost-effective, high-quality drug manufacturing.

43. Development of Immobilized Enzyme Membrane Bioreactors

Postgraduates can design enzyme-coated membranes for continuous hydrolysis of starch or cellulose. Reactor performance is evaluated for stability and productivity. This project links advanced reactor design with India’s food and biofuel industries.

44. Machine Learning Models for Predicting Enzyme Stability

Students can develop machine learning algorithms trained on enzyme sequence and structural data to predict stability under industrial conditions. Predictions are validated with laboratory assays. This project reflects India’s integration of AI into biotechnology research.

45. Enzymatic Valorization of Dairy Waste Whey into High-value Products

Postgraduates can apply proteases and lactases to convert whey into bioactive peptides and prebiotics. Functional properties like antioxidant or antimicrobial activity are analyzed. This project supports India’s massive dairy sector in moving toward zero-waste processing.

46. Fusion Enzyme Design for Multi-functional Catalysis

Students can construct fusion proteins combining cellulase and xylanase domains for efficient biomass hydrolysis. Fusion constructs are expressed and tested for synergistic activity. This project highlights protein engineering strategies now entering Indian enzyme research.

47. Application of Immobilized Enzyme Systems in Pharmaceutical Intermediates

Postgraduates can immobilize ketoreductases or transaminases for synthesis of pharmaceutical intermediates. Stability and yield are compared with free enzymes. This project supports India’s pharma sector, which is adopting biocatalysis to replace hazardous chemical synthesis.

48. Development of Enzyme-assisted Carbon Capture Technologies

Students can investigate carbonic anhydrase immobilization in reactors to accelerate CO₂ absorption. Efficiency is measured under simulated flue gas conditions. This project aligns with India’s carbon mitigation initiatives where biotech-based solutions are being explored.

49. Comparative Life Cycle Assessment (LCA) of Enzymatic vs. Chemical Processes

Postgraduates can conduct LCA studies comparing enzymatic starch hydrolysis, bio-bleaching, or biodiesel production with traditional chemical methods. Environmental and economic impacts are analyzed. This project supports India’s sustainable industrial policy framework.

50. Roadmap for Commercialization of Industrial Enzyme Biorefineries in India

Students can develop techno-economic models for integrated enzyme biorefineries producing enzymes, biofuels, and co-products. Case studies of Indian companies and policy analysis are included. This project provides strategic insights into India’s bioeconomy development.

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