Graduation Level Topics

1. Seroprevalence Survey of a Viral Antigen in a Local Population (ELISA-Based)
   Aim: estimate antibody prevalence to a chosen viral antigen in a community cohort. Approach: design sampling strategy and use validated immunoassays in a laboratory setting, with appropriate controls. Validation: compare seroprevalence to reported case data and assess assay quality metrics (sensitivity/specificity). Relevance: informs local public-health planning and vaccine outreach in Indian districts. Deliverable: protocol style report with sampling, assay selection and interpretation guidelines.

2. Comparative Study of Rapid Diagnostic Tests vs Laboratory Gold-Standard for a Common Infection
   Aim: evaluate field RDT performance against lab confirmatory tests on the same sample set. Approach: blind testing, calculation of predictive values across prevalence ranges and simple error analysis. Validation: ROC curves and confusion matrices to quantify utility in primary care. Relevance: helps district clinics choose appropriate point-of-care tests in India. Output: a practical evaluation protocol with decision thresholds.

3. Isolation and Characterization of Commensal Skin Bacteria with Antimicrobial Potential
   Aim: screen skin isolates for bacteriocin or inhibitory activity against opportunistic pathogens. Approach: isolate candidate strains, perform taxonomy via 16S sequence (conceptual) and document antimicrobial spectra. Validation: in-vitro inhibition profiling and basic safety assessment conceptually. Relevance: discovery of probiotic or topical biologic leads for dermatological use in India. Deliverable: characterization abstract and follow-up validation plan.

4. In-Silico Epitope Mapping of a Local Pathogen to Inform Diagnostic Design
   Aim: identify conserved peptide epitopes useful for antibody detection across circulating strains. Approach: use public sequences to predict B-cell epitopes and cross-reactivity risk. Validation: propose synthetic peptide validation in ELISA and cross-validation with clinical samples. Relevance: supports regionally-tuned diagnostics for India’s strain diversity. Output: computational protocol and experimental validation roadmap.

5. Survey of Antimicrobial Resistance Markers in Clinical Isolates — Data Analysis Project
   Aim: analyze resistance patterns from a hospital’s retrospective culture dataset to identify trends. Approach: collate antibiograms, perform temporal and ward-wise comparisons and simple statistical analysis. Validation: highlight priority organisms and resistance mechanisms for stewardship interventions. Relevance: actionable input for antibiotic policy at Indian hospitals. Deliverable: analysis plan and recommendations brief.

6. Development of a Low-Cost Sample Collection and Transport Kit for Rural Diagnostics
   Aim: design and evaluate a practical kit (non-technical description) to preserve clinical samples for centralized testing. Approach: prototype logistics, use stability data from literature and map cold-chain alternatives. Validation: model sample integrity across transport times and recommend SOPs for rural clinics. Relevance: increases diagnostic reach in India’s remote healthcare settings. Output: kit specification and field-validation protocol.

7. Comparative Analysis of DNA Extraction Methods from Buccal Swabs (Data/Method Focus)
   Aim: compare yields and inhibitor profiles from commonly used extraction concepts using archived data or literature. Approach: summarize pros/cons, cost, throughput and downstream PCR suitability. Validation: synthetic comparison tables and decision matrix for student labs. Relevance: practical guidance for resource-limited diagnostic labs in India. Deliverable: method selection guide and validation checklist.

8. Preliminary Evaluation of Probiotic Strains for Gastrointestinal Health (In Vitro Focus)
   Aim: shortlist probiotic candidates from cultured products for acid/bile tolerance and antagonism against pathogens. Approach: compile strain attributes, plan conceptual in-vitro assays and safety checks. Validation: recommend top strains for controlled animal or human pilot studies. Relevance: supports nutraceutical development using indigenous cultures in India. Output: selection protocol and regulatory considerations.

9. Designing a Hospital-Level Wastewater Surveillance Program for Pathogen Monitoring
   Aim: propose a surveillance framework to monitor viral or bacterial markers from hospital effluent. Approach: map sampling points, analytical techniques (PCR/immunoassay conceptual) and data-action triggers. Validation: pilot sampling plan and correlation with clinical caseload. Relevance: early warning and public health action in Indian cities. Deliverable: surveillance SOP and interpretation plan.

10. Assessment of Antigen Avidity Maturation Post-Infection vs Post-Vaccination (Data Project)
    Aim: compare antibody avidity indices between naturally infected and vaccinated cohorts using available datasets or literature. Approach: conceptualize avidity assay interpretation and construct comparative plots over time. Validation: statistical comparison and discussion of protective correlates. Relevance: vaccine policy insights for booster dosing in India. Output: data-driven protocol and conclusions.

11. Basic Protein Biomarker Panel Selection for Early Disease Detection (Literature + Design)
    Aim: identify a small panel of protein biomarkers for early detection of a target disease (e.g., TB or liver disease). Approach: literature curation, selection criteria and conceptual multiplex immunoassay design. Validation: propose pilot sample sets and performance thresholds. Relevance: targeted biomarker panels can improve primary care screening in India. Deliverable: panel proposal and validation roadmap.

12. Feasibility Study: Milk-Based Antibody Testing for Herd Health Surveillance
    Aim: evaluate bulk-milk antibody testing as a herd-level surveillance tool for selected livestock pathogens. Approach: sampling strategy, assay selection and threshold planning conceptually. Validation: modeling detection probability relative to within-herd prevalence. Relevance: low-cost surveillance for India’s dairy cooperatives. Output: pilot design and interpretation guidelines.

13. Data Project: Modeling Diagnostic Test Supply Needs During an Outbreak
    Aim: forecast diagnostic kit demand during a simulated outbreak in a district. Approach: use epidemiological curves and test-positivity assumptions to model procurement timelines. Validation: scenario analysis and sensitivity to test turnaround times. Relevance: aids logistics planning for Indian public healthcare authorities. Deliverable: model and procurement recommendations.

14. Evaluation of Dry-Blood Spot (DBS) Utility for Serology in Field Surveys
    Aim: assess DBS suitability for population serosurveys compared with venous serum (conceptual and literature synthesis). Approach: summarize stability, assay adaptation needs and expected biases. Validation: correction factors and pilot verification plan suggested. Relevance: pragmatic sampling for large Indian serosurveys. Output: protocol adaptation and QC considerations.

15. Design of an Institutional Quality Control Program for Immunoassays
    Aim: prepare a QC program template covering control materials, frequency and performance criteria for routine immunoassays. Approach: define acceptance ranges, corrective action flows and record-keeping. Validation: include proficiency testing plans and external QA linkages. Relevance: raises testing reliability in district labs across India. Deliverable: QC manual outline.

16. Comparative Study of Commercial Rapid Antigen Kits for Respiratory Pathogens
    Aim: perform a literature-based comparative evaluation of available RDTs with respect to sensitivity in symptomatic vs asymptomatic contexts. Approach: collate performance data, stratify by viral load proxies and summarize operational pros/cons. Validation: provide procurement guidance for clinics in India. Output: evidence table and selection criteria.

17. Survey: Use and Interpretation of Point-of-Care CRP Tests in Primary Care
    Aim: map how CRP rapid tests are used in antibiotic stewardship decisions among primary-care providers. Approach: questionnaire design and conceptual analysis methods for trends. Validation: propose decision algorithms integrating CRP thresholds and clinical criteria. Relevance: reduce antimicrobial misuse in Indian outpatient settings. Deliverable: survey protocol and stewardship recommendations.

18. Conceptual Design of a Small-Scale Recombinant Protein Expression Study (Dry-Lab Planning)
    Aim: plan expression strategy for a non-hazardous recombinant protein for teaching labs (vector/host selection, expression markers conceptually). Approach: design construct maps, predicted solubility considerations and purification options. Validation: propose analytical checks (SDS-PAGE, Western blot conceptual) and QC. Relevance: builds hands-on recombinant protein literacy for Indian graduates. Output: project abstract and resource list.

19. Preliminary Assessment of Antibody Cross-Reactivity in Endemic Regions
    Aim: evaluate risk of cross-reactivity in serology panels due to co-endemic infections. Approach: literature review and in-silico antigen comparison, propose orthogonal test algorithms. Validation: recommend confirmatory testing strategies and interpretation heuristics. Relevance: improves accuracy of serologic surveillance in India. Deliverable: testing algorithm proposal.

20. Designing a Pilot Program for Newborn Screening Using Immunoassays (Feasibility Study)
    Aim: outline a pilot newborn screening program for selected disorders using immunoassays and DBS. Approach: screening criteria, referral pathways and data management conceptual plan. Validation: projected detection rates, false positives, and cost per case detected. Relevance: scalable newborn health interventions in India. Output: pilot protocol and stakeholder matrix.

21. Assessment of Biobanking Logistics and Consent Frameworks for Clinical Samples
    Aim: draft an operational plan for a small institutional biobank covering consent, storage and access policies. Approach: template consent language, anonymization practices and sample retention schedules (conceptual). Validation: compliance checklist with local ethical norms and regulatory guidance. Relevance: supports translational research capacity building in Indian hospitals. Deliverable: biobank SOP outline and governance plan.

22. Comparative Review: Nucleic Acid Extraction Methods for Diagnostic PCR (Resource Focus)
    Aim: evaluate extraction approaches for throughput, inhibitor tolerance and cost for a small diagnostic lab. Approach: compile performance summaries and propose selection criteria. Validation: recommended workflows for different sample types common in India. Output: method selection whitepaper for lab managers.

23. Evaluation of Antibody Decay Rates in Post-Exposure Cohorts (Data-Analysis Project)
    Aim: model antibody waning using longitudinal serologic data or published curves to estimate seroreversion times. Approach: apply mixed-effects models and produce interval estimates for surveillance interpretation. Validation: sensitivity analyses by age, severity or vaccine status. Relevance: refines seroprevalence interpretation for Indian studies. Deliverable: modeling protocol and interpretation guidance.

24. Pilot Design: Using Saliva for Non-Invasive Antibody Surveillance
    Aim: propose and evaluate sample collection, assay adaptation and logistics for saliva-based serosurveys. Approach: review sample stability and assay sensitivity trade-offs and plan pilot comparison with serum. Validation: establish correction equations and field collection SOPs. Relevance: less invasive population screening in India. Output: pilot plan and QC checks.

25. Survey of Diagnostic Turnaround Times and Impact on Clinical Decisions in a Tertiary Hospital
    Aim: analyze how lab turnaround affects antimicrobial prescriptions or admission decisions using retrospective data. Approach: time-series or case-control conceptual analysis. Validation: propose process improvements and measureable KPIs for service improvement. Relevance: operational enhancements for Indian hospitals. Deliverable: audit protocol and improvement plan.

26. Designing a Small-Scale Clinical Sample Metadata Management Template
    Aim: prepare a metadata schema for clinical samples that balances research utility and privacy. Approach: define essential fields, de-identification steps and access controls. Validation: pilot with retrospective sample sets and refine. Relevance: good data governance accelerates research in Indian institutions. Output: metadata template and governance note.

27. Evaluation of Common Protein Stabilizers for Transportation of Clinical Reagents
    Aim: synthesize literature on reagent stabilization approaches to minimize cold-chain dependence. Approach: compare excipient classes and recommended storage profiles. Validation: practical guidance for reagent procurement and field deployment in rural India. Deliverable: reagent handling best-practice brief.

28. Small Project: Mapping Laboratory Accreditation Requirements and Gaps for a District Hospital
    Aim: assess readiness for ISO/ NABL accreditation and identify priority gaps. Approach: checklist audit, staff training needs assessment and resource mapping. Validation: roadmap to accreditation with milestones and cost estimates. Relevance: quality uplift for Indian district labs. Output: gap analysis and action plan.

29. Assessment of Diagnostic Algorithms Incorporating Rapid Tests and Clinical Criteria
    Aim: propose algorithms combining RDTs with clinical scoring for specific syndromes (e.g., febrile illness). Approach: decision-tree construction and anticipated error profiling. Validation: propose pilot implementation and outcome metrics. Relevance: pragmatic algorithms reduce lab overuse in India. Deliverable: algorithm and evaluation plan.

30. Design of a Clinical Sample Triage System for Overburdened Labs
    Aim: create a prioritization framework for sample processing during surges. Approach: triage criteria, sample grouping and turnaround targets conceptualization. Validation: proposed KPIs and simulation scenarios to stress-test triage. Relevance: surge preparedness for Indian diagnostic labs. Output: triage SOP outline.

31. Feasibility Analysis: Local Production of Lateral-Flow Membranes and Components
    Aim: survey possibility and supply chain needs for local manufacture of LFA strips. Approach: map component suppliers, quality control needs and investment scales. Validation: simple economic model and regulatory pathway sketch. Relevance: reduce import dependency for diagnostics in India. Deliverable: feasibility report.

32. Data Review: Biomarker Variability Across Age and Sex for a Routine Clinical Analyte
    Aim: analyze cohort data to derive reference intervals and covariate effects for a common marker. Approach: statistical partitioning and reference interval estimation. Validation: compare with published norms and recommend local reference ranges. Relevance: improves diagnostic accuracy across India’s diverse populations. Output: reference interval protocol.

33. Conceptual Design for a Small-Scale Point-of-Care Molecular Testing Hub
    Aim: outline hub-and-spoke model where district clinics send prepped samples to a centralized POC hub with rapid PCR capacity. Approach: logistics, QC and result reporting pathways conceptualization. Validation: expected turnaround improvements and cost sharing model. Relevance: raises diagnostic capacity in underserved Indian regions. Deliverable: hub design brief.

34. Simple Pilot: Community Awareness and Consent Practices for Biomedical Sample Collection
    Aim: evaluate community understanding and consent quality in outreach sampling projects. Approach: design informed-consent templates and feedback instruments (conceptual). Validation: pilot surveys and iterative refinement. Relevance: ethical sample collection for Indian community studies. Output: consent template and evaluation plan.

35. Exploratory Evaluation of Commercial ELISA Kit Lot-to-Lot Variability
    Aim: assess potential variability across kit lots using control panels and literature data. Approach: compare QC metrics, recalibration needs and storage effects conceptualization. Validation: recommendations for lab procurement and lot verification steps. Relevance: ensures reliable diagnostics in Indian labs. Deliverable: verification SOP outline.

36. Design of an Accessible Dashboard for Laboratory QA Metrics
    Aim: build requirements for a simple dashboard to monitor QC, turnaround, and error rates at a lab. Approach: data fields, visualization needs and alert thresholds conceptualization. Validation: pilot with retrospective lab data and staff feedback. Relevance: improves lab management in Indian hospitals. Output: dashboard spec and rollout plan.

37. Survey Study: Healthcare Worker Knowledge on Biosafety and Sample Handling
    Aim: assess baseline biosafety awareness and training gaps among clinical staff. Approach: questionnaire design and conceptual training module development. Validation: pre/post training assessment metrics. Relevance: foundational to safe sample handling across India. Deliverable: survey and training plan.

38. Pilot Proposal: Stool Antigen vs PCR for a Gastrointestinal Pathogen — Operational Comparison
    Aim: compare operational aspects (cost, TAT, sensitivity) of stool antigen tests vs PCR for a target pathogen. Approach: collate performance data and design pilot comparative study. Validation: decision matrix for primary care adoption in India. Output: pilot protocol and cost analysis.

39. Design of a Low-Complexity Protein Electrophoresis Interpretation Training Module
    Aim: create training materials for junior lab staff on reading protein gels and interpreting patterns. Approach: curated example sets, scoring rubric and assessment exercises. Validation: pre/post competency testing. Relevance: practical skill building for Indian diagnostic labs. Deliverable: training module outline.

40. Data Project: Estimating the Impact of Diagnostic Delay on Patient Outcomes (Retrospective Study)
    Aim: quantify effect sizes of diagnostic delays on treatment outcomes using hospital records. Approach: cohort matching and survival/ outcome analysis conceptually. Validation: sensitivity analyses and policy implications. Relevance: prioritizes investments to reduce delays in Indian care pathways. Output: study design and analytic plan.

41. Feasibility Study of Using Mobile Phones for Remote Test Result Reporting
    Aim: prototype SMS/app-based result reporting for rural sample collection points. Approach: data security considerations, user flows and pilot roll-out plan. Validation: measure reporting timeliness and user acceptability in pilot sites. Relevance: bridges urban–rural diagnostic gaps in India. Deliverable: implementation brief.

42. Conceptual Protocol: Dried-Plasma Spot Use for Therapeutic Drug Monitoring
    Aim: evaluate the potential of dried-plasma spots for TDM in low-resource settings. Approach: literature review of stability, extraction needs and assay adaptation. Validation: propose pilot correlation studies with venous plasma. Relevance: expand TDM reach for Indian patients on narrow-therapeutic drugs. Output: pilot proposal.

43. Design a Simple Audit Tool for Preanalytic Errors in a Clinical Lab
    Aim: create an audit checklist to identify common sample collection and labeling errors. Approach: define error categories, sampling plan and remediation steps. Validation: implement short pilot and measure error reduction. Relevance: improves sample integrity in Indian labs. Deliverable: audit tool and SOP.

44. Practical Review: Integrating Point-of-Care Ultrasound Findings with Lab Diagnostics
    Aim: map concordance and complementarity of POCUS and lab tests in specific clinical workflows (e.g., sepsis triage). Approach: conceptual algorithms combining imaging and labs for triage decisions. Validation: suggest pilot studies and metrics for outcome improvements. Relevance: rapid diagnostics integration in Indian emergency care. Output: algorithm and pilot plan.

45. Small-Scale Project: Characterizing Storage Effects on Common Clinical Reagents
    Aim: summarize stability profiles and recommend handling precautions for frequently used reagents. Approach: literature synthesis and vendor data collation. Validation: practical guidance for procurement, storage and QC for Indian labs. Deliverable: reagent stability handbook.

46. Designing an Ethical Framework for Using Residual Clinical Samples in Research
    Aim: draft consent, anonymization and governance templates for re-use of leftover clinical specimens. Approach: align with national ethics guidance and propose oversight measures. Validation: stakeholder consultations and pilot implementation. Relevance: enables translational research in Indian hospitals. Output: framework document.

47. Survey of Local Needs for Diagnostic Capacity Building in Subdistrict Clinics
    Aim: map diagnostic gaps, staffing and training needs in a set of subdistrict clinics. Approach: needs-assessment questionnaire and resource mapping. Validation: prioritized capacity building plan and cost estimates. Relevance: targeted improvements for rural Indian healthcare. Deliverable: needs report and action plan.

48. Design of a Protocol for Simple Turnaround Time Audits in Diagnostic Labs
    Aim: provide a standardized audit template for measuring TAT across common tests. Approach: define time-stamps, bottleneck analysis and corrective actions. Validation: pilot audit and process improvements. Relevance: enhances lab efficiency in Indian settings. Output: audit SOP.

49. Preliminary Assessment of Saliva vs Nasopharyngeal Sampling Acceptability in Community Surveys
    Aim: evaluate acceptability and logistics of saliva sampling compared to NP swabs via participant surveys. Approach: design questionnaire and sampling workflow concepts. Validation: pilot acceptability metrics and proposed sample usage guidelines. Relevance: improves community uptake for Indian surveillance. Deliverable: study plan and recommendations.

50. Practical Protocol Design: Simple Cost-Benefit Analysis of a New Diagnostic Test Adoption
    Aim: develop a framework to evaluate economic and clinical impacts of adopting a new test at a hospital. Approach: define cost inputs, outcome measures and decision thresholds. Validation: scenario analyses and sensitivity checks. Relevance: supports evidence-based procurement decisions in Indian healthcare. Output: cost-benefit template and example application.

Post-Graduation Level Topics

·  Development and Validation of a Multiplexed Immunoassay Panel for Sepsis Biomarkers
Aim: design a multiplex panel combining acute-phase proteins and cytokines to stratify sepsis severity. Approach: select candidate markers from literature, prototype bead-based assay design conceptually and outline analytical validation parameters. Validation: inter-site reproducibility, ROC analysis against clinical outcomes and longitudinal profiling. Relevance: improved triage tools for intensive care units in India. Deliverable: study plan with QC and clinical integration roadmap.

·  NGS-Based Pathogen Detection Pipeline for Clinical Metagenomics
Aim: implement and validate a shotgun metagenomics workflow for pathogen ID from blood or CSF samples. Approach: sample prep considerations, bioinformatics pipeline design and contamination controls conceptualization. Validation: compare performance to culture/PCR using clinical cohorts and set reporting thresholds. Relevance: addresses culture-negative infections in tertiary Indian hospitals. Output: validated pipeline blueprint and reporting standards.

·  Designing CRISPR-Based Rapid Diagnostics for Point-of-Care Use
Aim: develop an isothermal CRISPR-collateral cleavage assay for a high-priority pathogen with field-friendly readout. Approach: target selection, guide design strategies and assay multiplexing conceptually. Validation: LOD determination, clinical sample comparison and stability testing for Indian climates. Relevance: rapid, sensitive diagnostics for decentralized Indian healthcare. Deliverable: assay validation plan and regulatory pathway.

·  Systems Immunology to Identify Correlates of Protection after Vaccination
Aim: integrate serology, B-/T-cell repertoire sequencing and transcriptomics to define protective signatures. Approach: longitudinal cohort sampling, multi-omics integration and machine-learning biomarker identification. Validation: predictive model testing in independent cohorts and mechanistic follow-up. Relevance: refines vaccine strategies for Indian subpopulations. Output: protocols for translational immunomonitoring and model validation.

·  Engineered Therapeutic Antibodies: Fc-Engineering for Enhanced Effector Function
Aim: design Fc variants to optimize ADCC/ADCP for targeted therapy. Approach: in-silico Fc modifications, proposed in-vitro functional assays and developability assessment (conceptual). Validation: correlate Fc glycoform or mutation profiles with effector readouts and preclinical models. Relevance: accelerate therapeutic antibody development in Indian biotech. Deliverable: engineering and validation roadmap.

·  Single-Cell Transcriptomics of Tumor-Infiltrating Immune Cells as a Predictive Biomarker
Aim: apply scRNA-seq to tumor biopsies to identify immune cell states predictive of immunotherapy response. Approach: design sampling, library planning and computational pipelines for cell-type and state discovery. Validation: correlate signatures with clinical response and validate using bulk deconvolution on larger cohorts. Relevance: precision oncology tools for Indian cancer centers. Output: experimental and analysis protocol.

·  Metagenomic Surveillance of Hospital-Associated Pathogens and Resistance Determinants
Aim: implement a metagenomic monitoring program for ICU environments to detect emerging resistance genes. Approach: environmental sampling, sequencing depth planning and bioinformatics surveillance dashboard concept. Validation: action thresholds, outbreak detection sensitivity and integration with infection control. Relevance: strengthens hospital biosafety in Indian tertiary centers. Deliverable: surveillance SOP and response plan.

·  Development of a Gene-Editing Strategy for Correcting a Monogenic Disorder in a Model System
Aim: design prime/base editing approaches for a disease-causing point mutation, focusing on target selection and off-target risk assessment. Approach: computational editing outcome prediction, choice of delivery method (conceptual) and in-vitro validation roadmap. Validation: sequencing-based edit quantification, functional rescue assays and safety profiling. Relevance: preclinical pathway planning for genetic medicine in India. Output: non-operational development plan and regulatory considerations.

·  High-Throughput Antibody Discovery from Human B-Cell Repertoires Using Single-Cell Technologies
Aim: mine convalescent or vaccinated donors’ repertoires to isolate potent neutralizing antibodies. Approach: single-cell sorting, BCR sequencing, sequence clustering and candidate prioritization with computational affinity prediction. Validation: recombinant expression, binding and neutralization assays and lead selection. Relevance: local antibody therapeutics and diagnostics discovery for Indian pathogens. Deliverable: discovery pipeline and validation criteria.

·  Proteomics-Driven Biomarker Discovery for Early Cancer Detection
Aim: identify circulating protein signatures informative for early-stage cancer via mass spectrometry. Approach: discovery cohort proteomics, statistical feature selection and targeted SRM/PRM validation design. Validation: independent cohort validation and clinical utility assessment. Relevance: non-invasive early detection tools for Indian cancer burden. Output: discovery/validation workflows and sample size guidance.

·  AI-Assisted Interpretation of Clinical NGS Reports for Variant Classification
Aim: build ML models that assist variant pathogenicity interpretation using aggregated databases and phenotypic data. Approach: feature engineering, model training and explainability frameworks for clinician use. Validation: retrospective variant reclassification exercise and clinical concordance analysis. Relevance: supports genomic medicine scale-up in Indian clinics. Deliverable: model deployment and QC plan.

·  Development and Validation of a Multiplexed CRP/Procalcitonin Cytokine Panel for Sepsis Triage
Aim: design a multiplex immunoassay combining host biomarkers for early sepsis risk stratification. Approach: marker selection from systems studies, multiplex assay conceptualization and QC planning. Validation: prospective cohort testing, ROC analysis and cost-effectiveness modeling. Relevance: support triage in resource-constrained Indian hospitals. Output: clinical validation roadmap.

·  Therapeutic Vaccine Design Using Computational Epitope Prioritization for a Chronic Infection
Aim: identify T-cell epitopes with high population coverage and low escape potential for a therapeutic vaccine candidate. Approach: immunoinformatics, HLA coverage modelling and antigen construct conceptualization. Validation: in-vitro T-cell assays proposal and preclinical immunogenicity plans. Relevance: targeted immunotherapeutics for Indian endemic infections. Deliverable: epitope selection and preclinical validation plan.

·  Advanced Bioinformatics Pipeline for Host–Pathogen Dual RNA-Seq in Clinical Samples
Aim: develop workflows to simultaneously analyze pathogen and host transcription from clinical specimens. Approach: contamination handling, read separation, differential expression and pathway analyses conceptualization. Validation: benchmark with synthetic mixes and pilot clinical cohort. Relevance: mechanistic insights into infection biology for Indian research. Output: pipeline spec and validation metrics.

·  Designing a Clinical Trial Framework for Passive Immunotherapy (Polyclonal Ig) in an Indian Setting
Aim: outline a Phase II trial protocol for passive immunotherapy, focusing on endpoints, dosing rationale and safety monitoring. Approach: inclusion/exclusion criteria, efficacy/safety endpoints and statistical planning conceptualization. Validation: sample size calculation and interim analysis plans. Relevance: translational pathway for biologic therapeutics in India. Deliverable: trial protocol skeleton and regulatory checklist.

·  Spatial Transcriptomics to Map Immune Microenvironments in Infectious Lesions
Aim: apply spatial gene expression methods to biopsy specimens to resolve immune-pathogen interactions in situ. Approach: sample preservation needs, capture technology choices and analysis strategies conceptualized. Validation: correlate spatial signatures with clinical outcomes and immune cell phenotypes. Relevance: deep phenotyping for Indian infectious disease research. Output: experimental and analysis plan.

·  Development of an Ex Vivo Human Tissue Model to Study Drug Penetration and Host Response
Aim: design an organotypic explant model to assess therapeutics’ tissue penetration and local immune response. Approach: tissue sourcing, culture conditions and readout selection (cytokines, imaging) conceptualization. Validation: compare to clinical pharmacokinetic data and in-vivo correlates. Relevance: bridge between preclinical and clinical testing in India. Deliverable: model development and validation roadmap.

·  Engineering CAR-T Cell Therapy Targeting a Locally Prevalent Cancer Antigen — Conceptual Roadmap
Aim: map the translational steps from target validation to manufacturing SOPs and early-phase clinical testing for CAR-T approaches. Approach: antigen selection, construct design constraints, safety switches and GMP considerations conceptualized. Validation: preclinical efficacy/safety study designs and regulatory interactions plan. Relevance: enabling cell therapy pathways tailored for Indian oncology needs. Output: translational roadmap and risk mitigation plan.

·  Microfluidic Single-Cell Immune Profiling Platform Development for Clinical Samples
Aim: design a platform for capturing immune cell functional readouts at single-cell level from small clinical samples. Approach: integrate cell capture, stimulation and multiplex readouts conceptually. Validation: pilot benchmarking with PBMCs and clinical specimens. Relevance: high-resolution immunomonitoring for translational studies in India. Deliverable: platform specifications and validation plan.

·  Longitudinal Multi-Omics of Post-Infection Sequelae to Identify Therapeutic Targets
Aim: follow cohorts post-infection with integrated omics (transcriptome, proteome, metabolome) to identify pathways driving chronic sequelae. Approach: sampling schema, data integration frameworks and causal inference strategies. Validation: prioritize druggable targets and propose intervention trials. Relevance: addresses long-term burden of infections in India. Output: cohort and analysis protocol.

·  Design and Validation of a Digital PCR Assay for Low-Level Pathogen Detection
Aim: develop a dPCR assay concept for ultra-sensitive pathogen detection in critical samples. Approach: target selection, partitioning strategy and analytical sensitivity planning conceptualized. Validation: LOD, linearity and comparison with qPCR in clinical panels. Relevance: diagnostics for low-burden infections in Indian clinics. Deliverable: validation plan and QC thresholds.

·  Host Genetic Determinants of Severe Disease: GWAS Planning and Integration with Functional Assays
Aim: design a GWAS to identify host variants associated with severe clinical outcomes and plan functional follow-up. Approach: cohort power calculations, phenotype harmonization and downstream cell-based validation conceptualization. Validation: replication cohorts and mechanistic assays to confirm causality. Relevance: personalized risk stratification in Indian populations. Output: GWAS and validation roadmap.

·  Pharmacogenomics Implementation Framework for Rational Drug Dosing in India
Aim: develop implementation pathway for clinically actionable pharmacogenomic markers for selected drugs. Approach: evidence review, assay selection, clinical decision support integration and cost-effectiveness modeling. Validation: pilot rollout in targeted clinics and outcome monitoring. Relevance: safer, individualized therapy in Indian healthcare. Deliverable: implementation protocol.

·  Adaptive Clinical Trial Design Using Biomarker-Driven Endpoints for Immunotherapies
Aim: propose an adaptive trial structure that uses early immunological biomarkers to enrich patient arms. Approach: adaptive randomization methods, interim analyses and biomarker assay harmonization conceptualized. Validation: simulation studies to estimate power and operational feasibility. Relevance: efficient trials in resource-constrained Indian research settings. Output: trial design blueprint.

·  Host-Pathogen Protein-Protein Interaction Mapping to Identify Therapeutic Targets
Aim: use computational and experimental interaction prediction to map key host–pathogen interfaces. Approach: integrate structural, interactome and evolutionary data to prioritize targets. Validation: propose orthogonal assays (binding, functional) and small-molecule screening funnels. Relevance: identify new therapies for pathogens of Indian priority. Deliverable: prioritization and validation plan.

·  Designing an End-to-End Clinical Metabolomics Workflow for Diagnostic Biomarker Discovery
Aim: implement protocols for sample collection, LC-MS analysis and data workflows to discover metabolic biomarkers. Approach: QA/QC standards, normalization and multi-site harmonization conceptualization. Validation: discovery and targeted confirmation phases with clinical cohorts. Relevance: metabolic diagnostics for Indian metabolic disorders. Output: standardized metabolomics SOP and validation plan.

·  Engineering Vaccine Antigens for Broad HLA Coverage in Diverse Indian Populations
Aim: design antigen constructs optimized for T-cell epitope coverage across Indian HLA diversity. Approach: immunoinformatics, population coverage modelling and construct design conceptualization. Validation: ex-vivo T-cell assays across HLA-typed donors and immunogenicity studies. Relevance: inclusive vaccine design for heterogeneous Indian genetics. Deliverable: antigen design and validation roadmap.

·  Clinical Validation of Cell-Free DNA Assays for Minimal Residual Disease Detection
Aim: establish workflows to detect low-frequency tumor mutations in plasma for MRD monitoring. Approach: targeted NGS panel design, error-suppression strategies and longitudinal sampling schema. Validation: correlate ctDNA dynamics with imaging and relapse in longitudinal cohorts. Relevance: non-invasive cancer monitoring for Indian oncology settings. Output: validation plan and clinical integration strategy.

·  High-Content Screening to Repurpose Approved Drugs for Emerging Infectious Diseases
Aim: design a high-throughput cell-based phenotypic screen to identify repurposing candidates among approved drugs. Approach: assay selection, library curation and hit triage conceptualization. Validation: orthogonal mechanistic assays and in-vivo proof-of-concept plans. Relevance: rapid therapeutic options for outbreaks in India. Deliverable: screening pipeline and validation criteria.

·  Multiplexed Spatial Proteomics for Tumor Microenvironment Characterization
Aim: implement multiplex antibody panels for simultaneous spatial mapping of immune and tumor proteins. Approach: panel design, imaging and quantification methods conceptualized. Validation: correlate spatial features with clinical response and propose translational biomarkers. Relevance: precision oncology in Indian clinical research centers. Output: protocol and analysis roadmap.

·  Synthetic Biology Platform for Manufacturing Peptide Therapeutics in Yeast
Aim: design conceptual production pipelines for peptide drugs in yeast, balancing expression, cleavage and purification. Approach: pathway and host optimization planning and downstream purification concepts. Validation: pilot yield estimates, activity assays and regulatory considerations. Relevance: domestic biologics manufacturing capability in India. Deliverable: production and scale-up roadmap.

·  Longitudinal Immunoprofiling to Predict Autoimmune Disease Flares
Aim: identify immune signatures predictive of impending autoimmune flare using multi-parameter immunomonitoring. Approach: serial sampling, high-dimensional cytometry and machine learning predictive models. Validation: prospective testing and clinical decision thresholds. Relevance: improves management of autoimmune patients in India. Output: cohort and analytic design.

·  Design of a Rapid Antibody Neutralization Assay Using Pseudovirus Systems for BSL-2 Labs
Aim: establish validated pseudovirus neutralization workflows adaptable to Indian lab capacities. Approach: pseudotype selection, standard curve construction and assay harmonization conceptualization. Validation: comparison with live-virus neutralization and inter-lab concordance studies. Relevance: functional antibody assessment for vaccine and therapeutic evaluation in India. Deliverable: assay validation plan.

·  Host-Directed Therapeutics Discovery: Screening for Modulators of Host Pathways
Aim: identify small molecules that modulate host pathways exploited by pathogens to reduce disease severity. Approach: target prioritization, cell-based screens and pathway assays conceptualized. Validation: in-vivo efficacy models and safety profiles proposed. Relevance: broad-spectrum therapeutic strategies for Indian infectious disease burden. Output: discovery and validation strategy.

·  Development of a Standardized Pipeline for Clinical Proteogenomics Integration
Aim: integrate genomic and proteomic data for tumor characterization to guide therapy. Approach: sample processing standards, data integration methods and clinical reporting templates conceptualized. Validation: pilot cohort demonstrating added clinical value over genomics alone. Relevance: advanced diagnostics for Indian cancer care. Deliverable: pipeline and reporting standards.

·  Designing an Ethics and Governance Framework for Genomic Data Sharing in India
Aim: establish safer, consented frameworks enabling responsible genomic data sharing across institutions. Approach: consent models, data access tiers and audit mechanisms conceptualized. Validation: consultation with diverse stakeholders and pilot data sharing agreements. Relevance: fosters collaborative genomic research while protecting participants in India. Output: governance blueprint and consent templates.

·  Artificial Intelligence for Automated Pathology Slide Analysis in Resource-Limited Labs
Aim: develop AI pipelines for automated screening of common pathologies on histology slides to assist pathologists. Approach: dataset curation, model training and explainability frameworks conceptualized. Validation: prospective reader-assisted studies and regulatory acceptance planning. Relevance: augment pathology capacity in Indian hospitals. Deliverable: deployment and validation roadmap.

·  Translational Development of RNA Therapeutics: Delivery and Targeting Strategies
Aim: design delivery concepts for RNA therapeutics targeting a specific disease, balancing efficacy and safety. Approach: nanoparticle platform selection, targeting ligand concepts and preclinical testing schemas conceptualized. Validation: biodistribution, expression and toxicity modeling. Relevance: next-gen therapeutics pathway for Indian biotech. Output: translational plan and preclinical milestones.

·  Establishing a National Reference Genome Panel to Improve Clinical Variant Interpretation
Aim: propose design and composition of a population reference panel capturing Indian genetic diversity to enhance clinical variant annotation. Approach: sampling strategy, sequencing depth and data sharing models conceptualized. Validation: benchmarking variant frequency differences and impact on clinical interpretation. Relevance: improves diagnostic accuracy nationwide. Deliverable: panel design and implementation roadmap.

·  High-Throughput Screening for Immunomodulatory Natural Products from Traditional Medicine Sources
Aim: apply modern screening to identify immune-active compounds from ethnobotanical leads. Approach: library creation, phenotypic immune assays and hit validation conceptualized. Validation: mechanistic assays and in-vivo models for priority hits. Relevance: scientifically validates traditional therapeutics for Indian health needs. Output: screening cascade and validation plan.

·  Development of a Clinical Decision Support Tool Integrating Molecular Diagnostics
Aim: build a decision support system that interprets molecular diagnostic results and provides treatment guidance. Approach: knowledge base design, rule engines and clinician UI conceptualization. Validation: retrospective and prospective clinical validation with key performance metrics. Relevance: helps clinicians use complex molecular data in Indian settings. Deliverable: system spec and validation plan.

·  Establishing GMP-Compliant Small-Scale Manufacturing for Recombinant Vaccines
Aim: roadmap to set up a GMP-capable pilot facility for recombinant subunit vaccines at regional scale. Approach: facility design concepts, QC assays and regulatory submission sketches. Validation: process qualification steps and batch release criteria. Relevance: local vaccine production capacity for Indian needs. Output: facility and validation plan.

·  Pharmacokinetic/Pharmacodynamic Modeling for Biologics in Indian Patient Populations
Aim: develop population PK/PD models incorporating demographic and genetic covariates relevant to India. Approach: model structure selection, parameter estimation strategies and simulation plans. Validation: model qualification against clinical trial datasets and dosing guideline proposals. Relevance: optimized biologic dosing for Indian patients. Deliverable: modeling and validation roadmap.

·  High-Resolution Mapping of Immune Receptor Repertoires in Autoimmune Disease Cohorts
Aim: profile BCR/TCR repertoires to identify clonotypes associated with disease activity and antigen specificity. Approach: repertoire sequencing, clonotype analysis and antigen-mapping strategies conceptualized. Validation: correlation with disease state and functional assays for candidate clonotypes. Relevance: personalized immunomonitoring for autoimmune care in India. Output: cohort and analysis plan.

·  Development of Biosafety and Containment Guidelines for Clinical Gene-Editing Trials
Aim: draft best-practice containment, monitoring and environmental risk assessments for first-in-human gene-editing studies. Approach: hazard analysis, monitoring schedules and community engagement templates conceptualized. Validation: alignment with regulators and ethical approvals. Relevance: safe translation of cutting-edge therapies in India. Deliverable: guideline draft and checklist.

·  Microbiome Therapeutics: Rational Design of Defined Consortia for Gut Dysbiosis
Aim: design a minimal, defined bacterial consortium to restore healthy gut function in a target indication. Approach: strain selection based on functional traits, safety profiling and manufacture considerations conceptualized. Validation: preclinical efficacy and stability testing with proposed clinical trial plan. Relevance: microbiome interventions for Indian gastrointestinal disease burden. Output: consortium design and translational pathway.

·  Clinical Validation of Wearable Biosensors for Continuous Physiological Monitoring
Aim: evaluate wearable biosensor accuracy and clinical utility for vital signs and biochemical proxies in hospital and community settings. Approach: device selection, validation protocols and data integration frameworks conceptualized. Validation: comparative studies against gold standards and outcome impact assessment. Relevance: scalable remote monitoring solutions for Indian healthcare. Deliverable: validation and integration plan.

·  Design of a National Biothreat Early-Warning System Integrating Genomics
Aim: propose an integrated surveillance network using environmental genomics, clinical sequencing and data analytics to detect emerging threats. Approach: sampling network design, data pipelines and legal/privacy frameworks conceptualized. Validation: simulated detection exercises and stakeholder engagements. Relevance: strengthen national preparedness in India. Output: system architecture and pilot plan.

·  Translational Pipeline for Developing Monoclonal Antibodies Against Regional Pathogens
Aim: map the pipeline from antigen selection to IND-enabling studies for mAbs targeting India-relevant pathogens. Approach: discovery, humanization, manufacturability, safety and regulatory roadmap conceptualized. Validation: go/no-go criteria at each stage and milestone planning. Relevance: build indigenous therapeutic antibody capacity. Deliverable: end-to-end development roadmap.

·  Policy & Implementation Study: Equitable Access to Advanced Molecular Diagnostics Across India
Aim: design policy proposals and implementation strategies to expand access to advanced diagnostics (NGS, proteomics) equitably. Approach: needs assessment, financing models, capacity building and regulatory harmonization conceptualized. Validation: pilot rollouts, monitoring equity metrics and cost-effectiveness analysis. Relevance: ensures advanced diagnostics benefit diverse Indian populations. Output: policy brief and phased implementation plan.